EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-9 Activity Tracking
Pricing (select from Available Options, above):
Trial size (25 assays) $159
96 assays $599
The EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-9 Activity Tracking is a fluorescence based protease activity detection kit for use with live three-dimensional (3D) cell culture or co-culture. The kit provides non-toxic, protease-selective substrate and far-red fluorescent dye to be mixed directly into invasion matrix / scaffold / hydrogel (e.g. Matrigel®, or collagen gel), enabling real-time monitoring of specific protease activity by fluorescent microscopy. Kit components are stable for at least six days in culture, and highly resistant to photobleaching. Custom substrate development also available. Excitation 625-635nm/ Emission 655-665nm. Cy5 or similar filters can also be used.
Imaging the activity of a specific protease in live cells: The micrographs below demonstrate fluorescent live-cell 3D imaging of protease activity (in this case using the EnSens® MMP-14 Activity Detection Substrate) in an invading MDA-MB-231/fibroblast spheroid. Spheroids were formed in the wells of a Corning® 96-well Spheroid Microplate, then surrounded with a layer of Matrigel® Matrix. Data were generated by BioTek® Instruments using their CytationTM 5 Cell Imaging Multi-mode Reader (brightfield and Cy5 channels) and Gen5TM data analysis software.
Selectivity of the EnSens® MMP-9 Activity Detection Substrate: In the graph below, left, the EnSens® MMP-9 activity detection substrate is cleaved extremely well by MMP-9, but not by other MMPs except MMP-2. Because the EnSens® MMP-2 substrate (right graph) is not cleaved by MMP-9, the two kits can be used in conjunction to obtain selectivity in mixture samples.
•EnSens® MMP-9 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
(MMP-9, 92 kDa type IV collagenase, gelatinase B, 92 kDa gelatinase, collagenase type IV-B, macrophage gelatinase, matrix metalloprotease-9)
MMP-9 is a ubiquitous metalloproteinase that is involved in diverse functions such as angiogenesis, tissue repair, tumor invasion and metastasis, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins such as collagen, elastin, and laminin, it can also act on non-matrix proteins such as pro-TNF-alpha, and interleukins and their receptors. It also cleaves ICAM-1 (Intercellular Adhesion Molecule-1), increasing tumor cell resistance to immune surveillance.
EnSens Fluorescent 3D Live Cell Imaging Kit for MMP-9 Activity Tracking
- Product Code: 022315_02
- Availability: In Stock