EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-14 Activity Tracking
Pricing (select from Available Options, above):
Trial size (25 assays) $159
96 assays $599
The EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-14 Activity Tracking is a fluorescence based protease activity detection kit for use with live three-dimensional (3D) cell culture or co-culture. The kit provides non-toxic, protease-selective substrate and far-red fluorescent dye to be mixed directly into invasion matrix / scaffold / hydrogel (e.g. Matrigel®, or collagen gel), enabling real-time monitoring of specific protease activity by fluorescent microscopy. Kit components are stable for at least six days in culture, and highly resistant to photobleaching. Custom substrate development also available. Excitation 625-635nm/ Emission 655-665nm. Cy5 or similar filters can also be used.
Imaging the activity of a specific protease in live cells: The micrographs below demonstrate fluorescent live-cell 3D imaging of MMP-14 protease activity in an invading MDA-MB-231/fibroblast spheroid. Spheroids were formed in the wells of a Corning® 96-well Spheroid Microplate, then surrounded with a layer of Matrigel® Matrix. Data were generated by BioTek® Instruments using their CytationTM 5 Cell Imaging Multi-mode Reader (brightfield and Cy5 channels) and Gen5TM data analysis software.
Selectivity of the EnSens® MMP-14 Activity Detection Substrate: Unlike ‘selective’ FRET substrates, the EnSens® MMP-14 activity detection substrate is cleaved extremely well by MMP-14, but not by other MMPs (left graph). The graphs on the right, generated by the Core Molecular Screening Facility at The Wistar Institute, demonstrate that a ‘selective’ FRET-based MMP-14 substrate is actually cleaved by MMP-2 quite well.
•EnSens® MMP-14 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
(MMP-14, Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP, MT-MMP1, matrix metalloprotease-14)
A membrane-inserted metalloproteinase that can also be released as a soluble active form, MMP-14 is involved in diverse functions such as arthritis, angiogenesis, tissue repair, tumor invasion and metastasis, and inflammation. In addition to degrading extracellular matrix proteins such as collagen, gelatin, and aggrecan, it can also act on non-matrix proteins such as CD44 and CCL2 (Monocyte chemoattractant protein-1). It also cleaves and activates its family member, MMP-2, further enhancing tumor invasion.
EnSens Fluorescent 3D Live Cell Imaging Kit for MMP-14 Activity Tracking
- Product Code: 022315_03
- Availability: In Stock