EnSens® Thrombin Activity Detection Kit Description
Pricing (select from Available Options, above):
Trial size (25 assays) $156
96 assays $625
384 assays $975
Also available through VWR
Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.
Selectivity of the EnSens® Thrombin Activity Detection Substrate: Despite the highly-overlapping substrate specificities of Thrombin and Factor Xa, EnSens® substrates are able to differentiate between the two enzymes. In the graph below, the EnSens® Thrombin activity detection substrate is cleaved extremely well by Thrombin, but not at all by Factor Xa.
•EnSens® Thombin Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water
(Coagulation factor IIa, fibrinogenase)
A clotting cascade protease initially expressed as inactive prothrombin and activated by factor Xa, thrombin cleaves fibrinogen at Arg-Gly, generating insoluble fibrin and fibrinopeptides A and B. It also cleaves and activates other clotting factor proteases, as well as clotting cascade inhibitor protein C. Thrombin is inactivated by the serpin antithrombin, and it is the target of the drug dabigatran (Pradaxa/Pradax/Prazaxa).
Together, thrombin and factor Xa proteases play prominent roles in the coagulation cascade and are major targets for emerging anti-coagulant therapies and associated diagnostics. EnSens fluorimetric reagents enable selective tracking and quantification of Factor Xa and Thrombin activation in purified protease, plasma, and whole blood samples.
EnSens Thrombin Activity Detection Kit
- Product Code: 032014_06
- Availability: In Stock