EnSens® MMP-25 Activity Detection Kit Description
Pricing (select from Available Options, above):
Trial size (25 assays) $171
96 assays $685
384 assays $1050
Also available through VWR
Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.
Selectivity of the EnSens® MMP-25 Activity Detection Substrate: The EnSens® MMP-25 activity detection substrate demonstrates how EnSens® technology can generate new, selective protease substrates. Until now there were no known MMP-25 substrates, but the EnSens® MMP-25 substrate is cleaved extremely well by MMP-25 (kcat/Km 1.25 x 105 M-1s-1). It is also cleaved quite well by MMP-17 (MT4-MMP), likely because they are extremely closely related enzymes, being the only GPI (glycosyl-phosphatidylinositol)-anchored membrane-type MMPs, with very similar substrate specificities. However, note the selectivity of the MMP-25 substrate against the remaining MT-MMPs (MMP-14/MT1-MMP, MMP-15/MT2-MMP, MMP-16/MT3-MMP, MMP-24/MT5-MMP).
•EnSens® MMP-25 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water
(MMP-25, Membrane-Type 6 Matrix Metalloproteinase, MT6-MMP, MT-MMP6, matrix metalloprotease-25, leukolysin)
A membrane-anchored metalloproteinase that can also be released as a soluble active form, MMP-25 is involved in diverse functions such as periodontal disease, arthritis, angiogenesis, tumor invasion and metastasis, and asthma. In addition to degrading extracellular matrix proteins such as collagen, fibronectin, and vimentin, it can also act on non-matrix proteins such as alpha 1 antitrypsin (mediating neutrophil migration) and chemokines. It also cleaves and activates its family member, MMP-2, further enhancing tumor invasion.
EnSens MMP-25 Activity Detection Kit
- Product Code: 032014_04
- Availability: In Stock