EnSens® Fluorescent 3D Live Cell Imaging Kit for ADAM17 Activity Tracking
Pricing (select from Available Options, above):
Trial size (25 assays) $159
96 assays $599
The EnSens® Fluorescent 3D Live Cell Imaging Kit for ADAM17 Activity Tracking is a fluorescence based protease activity detection kit for use with live three-dimensional (3D) cell culture or co-culture. The kit provides non-toxic, protease-selective substrate and far-red fluorescent dye to be mixed directly into invasion matrix / scaffold / hydrogel (e.g. Matrigel®, or collagen gel), enabling real-time monitoring of specific protease activity by fluorescent microscopy. Kit components are stable for at least six days in culture, and highly resistant to photobleaching. Custom substrate development also available. Excitation 625-635nm/ Emission 655-665nm. Cy5 or similar filters can also be used.
Imaging the activity of a specific protease in live cells: The micrographs below demonstrate fluorescent live-cell 3D imaging of protease activity (in this case using the EnSens® MMP-14 Activity Detection Substrate) in an invading MDA-MB-231/fibroblast spheroid. Spheroids were formed in the wells of a Corning® 96-well Spheroid Microplate, then surrounded with a layer of Matrigel® Matrix. Data were generated by BioTek® Instruments using their CytationTM 5 Cell Imaging Multi-mode Reader (brightfield and Cy5 channels) and Gen5TM data analysis software.
Selectivity of the EnSens® ADAM17 Activity Detection Substrate: Despite the highly-overlapping substrate specificities of ADAM17 and ADAM10, EnSens® substrates are able to differentiate between the two enzymes.
•EnSens® ADAM17 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
A DISINTEGRIN AND METALLOPROTEASE 17
(ADAM17, TACE, TNF-alpha Converting Enzyme, TNF-alpha convertase, CD156b, A Disintegrin And Metalloproteinase 17)
ADAM17/TACE is the major TNF-alpha (tumor necrosis factor alpha) sheddase. It can be either soluble or membrane-bound, and also cleaves Notch receptors, amyloid precursor protein, L-selectin, cadherins, ACE2 (angiotensin converting enzyme 2), EGFR, and TGF-alpha (transforming growth factor alpha). Thus it is involved in many important processes and diseases, such as development, inflammation, arthritis, cancer, Alzheimer’s disease, Crohn’s disease, obesity, and atherosclerosis.
EnSens Fluorescent 3D Live Cell Imaging Kit for ADAM17 Activity Tracking
- Product Code: 022315_09
- Availability: In Stock