EnSens® Technology

EnSens® technology is based on a proprietary fluorescent molecular rotor dye, which does not fluoresce when free in solution. The other component of EnSens® technology is its substrates, which are recombinant (E. coli) proteins comprising two domains. The substrate’s activation domain binds the molecular rotor dye, while the inhibitory domain interacts with the activation domain, blocking dye binding. The two domains are connected by a flexible linker that includes a protease cleavage recognition site, which can be as long or short as needed to impart selectivity. This modular nature enables rapid development of novel and selective protease substrates. When cleavage by the target protease separates the two domains, the activation domain dimerizes and binds the dye, causing it to fluoresce brightly (Ex./Em. 635/656 nm).

How EnSens Technology Works

EnSens® Technology Advantages 

  • Selective: Target specific protease of interest; substrate module can accommodate up to 200 amino acid protease recognition sequences
  • Versatile: Modular, Lego™-like, protein-based substrates enable us to easily customize to your favorite protease
  • Sensitive and Quantitative at picomolar levels of active protease. Repeatable, Reproducible, and Robust
  • Fewer False Positives and Negatives: 6-30X signal-to-noise ratios; red wavelengths result in greater sensitivity and accuracy
  • Cell Based Assays: Stable, protein-based (scFv) substrate & nontoxic dye allow cell surface detection of protease activity
  • Bead Based Assays allow for multiplexing in flow cytometry and other applications
  • HTS and HCS—EnSens® substrates are naturally resistant to degradation
  • High Signal Stability, resistant to photobleaching over 48 hours
  • Easy: Mix and Read. No antibodies, no special equipment or complicated data analysis

EnSens® kits currently available: Protease Activity Detection Kits and 3D Live Cell Imaging Kits for Protease Activity Tracking Kits

Applications for EnSens® Protease Substrates

  • Microplate Assays miniaturized to 5 uL total assay volume
  • Flow Cytometry and Cell Sorting
  • in situ protease activity imaging
  • in vivo protease activity imaging
  • HTS and HCS: inhibitor screening, phenotypic screening, activity-based profiling, off-target effects
  • 3D matrix / live-cell imaging studies
  • Biopsy tissue biomarker identification
  • Bead based assays
  • Multiplexing
  • Biofluid testing: tissue/cell lysate, serum, plasma, synovial fluid, sputum, urine
  • Protease substrate library screening

Let us know how you would like to use our products. We’ll work with you to develop the appropriate protocol.

[email protected]

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