EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-2 Activity Tracking
Pricing (select from Available Options, above):
Trial size (25 assays) $159
96 assays $599
The EnSens® Fluorescent 3D Live Cell Imaging Kit for MMP-2 Activity Tracking is a fluorescence based protease activity detection kit for use with live three-dimensional (3D) cell culture or co-culture. The kit provides non-toxic, protease-selective substrate and far-red fluorescent dye to be mixed directly into invasion matrix / scaffold / hydrogel (e.g. Matrigel®, or collagen gel), enabling real-time monitoring of specific protease activity by fluorescent microscopy. Kit components are stable for at least six days in culture, and highly resistant to photobleaching. Custom substrate development also available. Excitation 625-635nm/ Emission 655-665nm. Cy5 or similar filters can also be used.
Imaging the activity of a specific protease in live cells: The micrographs below demonstrate fluorescent live-cell 3D imaging of protease activity (in this case using the EnSens® MMP-14 Activity Detection Substrate) in an invading MDA-MB-231/fibroblast spheroid. Spheroids were formed in the wells of a Corning® 96-well Spheroid Microplate, then surrounded with a layer of Matrigel® Matrix. Data were generated by BioTek® Instruments using their CytationTM 5 Cell Imaging Multi-mode Reader (brightfield and Cy5 channels) and Gen5TM data analysis software.
Selectivity of the EnSens® MMP-2 Activity Detection Substrate: In the graph below, left, the EnSens® MMP-2 activity detection substrate is cleaved extremely well by MMP-2, but not at all by closely-related MMP-9, nor other MMPs except MMP-7 and MMP-13. Because the EnSens® MMP-9 substrate (right graph) is not cleaved by other MMPs except MMP-2, the two kits can be used in conjunction to obtain selectivity in mixture samples.
•EnSens® MMP-2 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
(MMP-2, 72 kDa type IV collagenase, gelatinase A, 72 kDa gelatinase, collagenase type IV-A, neutrophil gelatinase, matrix metalloproteinase-II, matrix metalloprotease-2)
MMP-2 is a ubiquitous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins, it can also act on several non-matrix proteins such as big endothelial 1 and beta-type CGRP, promoting vasoconstriction. Also cleaves KISS at a Gly-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
EnSens Fluorescent 3D Live Cell Imaging Kit for MMP-2 Activity Tracking
- Product Code: 022315_01
- Availability: In Stock