• EnSens Thrombin Activity Detection Kit

EnSens® Thrombin Activity Detection Kit Description

Pricing (select from Available Options, above):
Trial size (25 assays) $156
96 assays $625
384 assays $975

Also available through VWR

Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.

Selectivity of the EnSens® Thrombin Activity Detection Substrate: Despite the highly-overlapping substrate specificities of Thrombin and Factor Xa, EnSens® substrates are able to differentiate between the two enzymes. In the graph below, the EnSens® Thrombin activity detection substrate is cleaved extremely well by Thrombin, but not at all by Factor Xa.

EnSens Thrombin Substrate Selectivity

 

 

 

 

 

 

 


Kit Components:
•EnSens® Thombin Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water

THROMBIN
(Coagulation factor IIa, fibrinogenase)
EC 3.4.21.5

BIOLOGICAL ROLES
A clotting cascade protease initially expressed as inactive prothrombin and activated by factor Xa, thrombin cleaves fibrinogen at Arg-Gly, generating insoluble fibrin and fibrinopeptides A and B. It also cleaves and activates other clotting factor proteases, as well as clotting cascade inhibitor protein C. Thrombin is inactivated by the serpin antithrombin, and it is the target of the drug dabigatran (Pradaxa/Pradax/Prazaxa).

Together, thrombin and factor Xa proteases play prominent roles in the coagulation cascade and are major targets for emerging anti-coagulant therapies and associated diagnostics. EnSens fluorimetric reagents enable selective tracking and quantification of Factor Xa and Thrombin activation in purified protease, plasma, and whole blood samples.

EnSens Thrombin Activity Detection Kit

  • Product Code: 032014_06
  • Availability: In Stock
  • $625.00


Available Options


Related Products

EnSens Factor Xa Activity Detection Kit

EnSens Factor Xa Activity Detection Kit

EnSens® Factor Xa Activity Detection Kit Description Pricing (select from Available Options, abov..

$675.00

EnSens Fluorescent 3D Live Cell Imaging Kit for Factor Xa Activity Tracking

EnSens Fluorescent 3D Live Cell Imaging Kit for Factor Xa Activity Tracking

EnSens® Fluorescent 3D Live Cell Imaging Kit for Factor Xa Activity Tracking Pricing (select from..

$599.00

EnSens Fluorescent 3D Live Cell Imaging Kit for Thrombin Activity Tracking

EnSens Fluorescent 3D Live Cell Imaging Kit for Thrombin Activity Tracking

EnSens® Fluorescent 3D Live Cell Imaging Kit for Thrombin Activity Tracking Pricing (select from ..

$599.00

Tags: thrombin, protease, enzyme, fluorescence, activity detection, reaction buffer, coagulation