• EnSens MMP-9 Activity Detection Kit

EnSens® MMP-9 Activity Detection Kit Description

Pricing (select from Available Options, above):
Trial size (25 assays) $169
96 assays $675
384 assays $1039

Also available through VWR

Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.

Selectivity of the EnSens® MMP-9 Activity Detection Substrate: In the graph below, left, the EnSens® MMP-9 activity detection substrate is cleaved extremely well by MMP-9, but not by other MMPs except MMP-2. Because the EnSens® MMP-2 substrate (right graph) is not cleaved by MMP-9, the two kits can be used in conjunction to obtain selectivity in mixture samples.

EnSens MMP-9 Substrate SelectivityEnSens MMP-2 Substrate vs. MMP-9 Enzyme

 

 

 

 

 

 




Kit Components:
•EnSens® MMP-9 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water

MATRIX METALLOPROTEINASE-9
(MMP-9, 92 kDa type IV collagenase, gelatinase B, 92 kDa gelatinase, collagenase type IV-B, macrophage gelatinase, matrix metalloprotease-9)
EC 3.4.24.35

BIOLOGICAL ROLES
MMP-9 is a ubiquitous metalloproteinase that is involved in diverse functions such as angiogenesis, tissue repair, tumor invasion and metastasis, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins such as collagen, elastin, and laminin, it can also act on non-matrix proteins such as pro-TNF-alpha, and interleukins and their receptors. It also cleaves ICAM-1 (Intercellular Adhesion Molecule-1), increasing tumor cell resistance to immune surveillance.

EnSens MMP-9 Activity Detection Kit

  • Product Code: 032014_02
  • Availability: In Stock
  • $675.00


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Tags: MMP-9, gelatinase, protease, enzyme, fluorescence, activity detection, reaction buffer, metalloproteinase