• EnSens MMP-2 Activity Detection Kit

EnSens® MMP-2 Activity Detection Kit Description

Pricing (select from Available Options, above):
Trial size (25 assays) $175
96 assays $700
384 assays $1092

Also available through VWR

Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.

Selectivity of the EnSens® MMP-2 Activity Detection Substrate: In the graph below, left, the EnSens® MMP-2 activity detection substrate is cleaved extremely well by MMP-2, but not at all by closely-related MMP-9, nor other MMPs except MMP-7 and MMP-13. Because the EnSens® MMP-9 substrate (right graph) is not cleaved by other MMPs except MMP-2, the two kits can be used in conjunction to obtain selectivity in mixture samples.

EnSens MMP-2 Substrate SelectivityEnSens MMP-9 Substrate Selectivity

 

 

 

 

 

 

 


Kit Components:
•EnSens® MMP-2 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water

MATRIX METALLOPROTEINASE-2
(MMP-2, 72 kDa type IV collagenase, gelatinase A, 72 kDa gelatinase, collagenase type IV-A, neutrophil gelatinase, matrix metalloproteinase-II, matrix metalloprotease-2)
EC 3.4.24.24

BIOLOGICAL ROLES
MMP-2 is a ubiquitous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins, it can also act on several non-matrix proteins such as big endothelial 1 and beta-type CGRP, promoting vasoconstriction. Also cleaves KISS at a Gly-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.

EnSens MMP-2 Activity Detection Kit

  • Product Code: 032014_01
  • Availability: In Stock
  • $700.00


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Tags: MMP-2, gelatinase, protease, enzyme, fluorescence, activity detection, reaction buffer, metalloproteinase