• EnSens MMP-14 Activity Detection Kit

EnSens® MMP-14 Activity Detection Kit Description

Pricing (select from Available Options, above):
Trial size (25 assays) $175
96 assays $700
384 assays $1092

Also available through VWR

Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.

Selectivity of the EnSens® MMP-14 Activity Detection Substrate: Unlike ‘selective’ FRET substrates, the EnSens® MMP-14 activity detection substrate is cleaved extremely well by MMP-14, but not by other MMPs (left graph). The graphs on the right, generated by the Core Molecular Screening Facility at The Wistar Institute, demonstrate that a ‘selective’ FRET-based MMP-14 substrate is actually cleaved by MMP-2 quite well.

EnSens MMP-14 Substrate SelectivityEnSens vs. FRET

 

 


 

 

 

 

 

Kit Components:
•EnSens® MMP-14 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water

MATRIX METALLOPROTEINASE-14
(MMP-14, Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP, MT-MMP1, matrix metalloprotease-14)
EC 3.4.24.80

BIOLOGICAL ROLES
A membrane-inserted metalloproteinase that can also be released as a soluble active form, MMP-14 is involved in diverse functions such as arthritis, angiogenesis, tissue repair, tumor invasion and metastasis, and inflammation. In addition to degrading extracellular matrix proteins such as collagen, gelatin, and aggrecan, it can also act on non-matrix proteins such as CD44 and CCL2 (Monocyte chemoattractant protein-1). It also cleaves and activates its family member, MMP-2, further enhancing tumor invasion.

EnSens MMP-14 Activity Detection Kit

  • Product Code: 032014_03
  • Availability: In Stock
  • $700.00


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Tags: MMP-14, mt-mmp1, mt1-mmp, protease, enzyme, fluorescence, activity detection, reaction buffer, metalloproteinase