• EnSens MMP-3/MMP-10 Activity Detection Kit

EnSens® MMP-3/MMP-10 Activity Detection Kit Description

Pricing (select from Available Options, above):
Trial size (25 assays) $169
96 assays $675
384 assays $1039

Also available through VWR

Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.

Selectivity of the EnSens® MMP-13 Activity Detection Substrate: Despite the highly-overlapping substrate specificities of MMPs, EnSens® substrates are designed for enhanced selectivity. The graph shows that at pH 6.0, the EnSens® MMP-3/MMP-10 activity detection substrate is resistant to most MMPs, although it is cleaved by MMP-2, MMP-9, and MMP-13 in addition to MMP-3 and MMP-10 (light blue bars). Simple addition of the commercially available inhibitor SB-3CT to the assay makes it selective for MMP-3 and MMP-10 (dark blue bars).

EnSens MMP-3/MMP-10 Substrate Selectivity

 

 

 

 

 

 





Kit Components:
•EnSens® MMP-3/MMP-10 Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water

MATRIX METALLOPROTEINASE-3 and MATRIX METALLOPROTEINASE-10
(MMP-3, stromelysin-1, SL-1, transin-1;  MMP-10, stromelysin-2, SL-2, transin-2)
EC 3.4.24.17 (MMP-3) and 3.4.24.22 (MMP-10)

BIOLOGICAL ROLES
MMP-3 and MMP-10 are both known as stromelysins and possess significant overlap in their substrate recognition profiles. (MMP-11 is the third stromelysin and recognizes a largely unique set of substrates.) MMP-3 and MMP-10 are involved in diverse functions such as wound and muscle repair, infection, cancer, stroke, and arthritis. In addition to degrading extracellular matrix and basement membrane proteins such as gelatin, laminin, aggrecan, and fibronectin, they can also activate pro-MMPs. Other known targets for MMP-3 are decorin, IL-1beta, and pro-TNF-alpha.

EnSens MMP-3/MMP-10 Activity Detection Kit

  • Product Code: 032014_10
  • Availability: In Stock
  • $675.00


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Tags: MMP-3, MMP-10, stromelysin, protease, enzyme, fluorescence, activity detection, reaction buffer, metalloproteinase