EnSens® Literature

Brochures Application Notes Technical Notes

EnSens® Protease Assays

MMP Selectivity Application Note

Fluorimetry Optimization Suggestions

EnSens® MMP Selectivity

Drug Discovery Application Note
 

EnSens® Protease Drug Discovery

3D MMP Activity Application Note
 

EnSens® vs. FRET/AMC Protease Substrates

   


Scientific Literature References for EnSens® Technology

EnSens® Fluorescent Protease Activity Detection Substrates are also referred to as FAPs (Fluorogen Activating Proteins), or Fluoromodules. FAPs can be used as labels, or as protease substrates (EnSens®), in any fluorescent technology, including fluorometry, live-cell imaging, and flow cytometry.

FAPs as Protease Substrates
Bachert C, Linstedt AD. A sensor of protein O-glycosylation based on sequential processing in the Golgi apparatus. Traffic 2013 14: 47-56.

FAPs as Labels
Wu Y, Tapia PH, Jarvik J, Waggoner AS, Sklar LA. Real-time detection of protein trafficking with high-throughput flow cytometry (HTFC) and fluorogen-activating protein (FAP) base biosensor. Curr Protoc Cytom. 2014 67: Unit 9.43.
Yates BP, Peck MA, Berget PB. Directed evolution of a fluorogen-activating single chain antibody for function and enhanced brightness in the cytoplasm. Mol Biotechnol. 2013 54: 829-41.
Saunders MJ et al. Fluorogen activating proteins in flow cytometry for the study of surface molecules and receptors. Methods 2012 57: 308-17.
Fitzpatrick JA et al. STED nanoscopy in living cells using Fluorogen Activating Proteins. Bioconjug Chem. 2009 20: 1843-1847.
Szent-Gyorgyi C et al. Fluorogen-activating single-chain antibodies for imaging cell surface proteins. Nat Biotechnol. 2008 26: 235-40.

FAP Development
Szent-Gyorgyi C et al. Malachite green mediates homodimerization of antibody VL domains to form a fluorescent ternary complex with singular symmetric interfaces. J Mol Biol. 2013 425: 4595-613.
Falco CN, Dykstra KM, Yates BP, Berget PB. scFv-based fluorogen activating proteins and variable domain inhibitors as fluorescent biosensor platforms. Biotechnol J. 2009 4: 1328-36.