EnSens® Factor Xa Activity Detection Kit Description
Pricing (select from Available Options, above):
Trial size (25 assays) $169
96 assays $675
384 assays $1030
Also available through VWR
Mix-and-Read, Fluorescence based Protease Activity detection kit. The kit includes protease selective detection reagent, fluorescent dye, reaction buffer, and substrate positive control. Protocol volume can be easily scaled up or down as needed. It is designed for fluorimetric endpoint and kinetic microplate assays. Quantifiable down to nano- and picomolar range of active protease concentrations. Upon activation, se-Red reagent produces detectable fluorescent signal in the far red region of the light spectrum, reducing background signal and increasing dynamic range. Excitation 625-635nm/ Emission 655-665nm.
Selectivity of the EnSens® Factor Xa Activity Detection Substrate: Despite the highly-overlapping substrate specificities of Factor Xa and Thrombin, EnSens® substrates are able to differentiate between the two enzymes. In the graph below, the EnSens® Factor Xa activity detection substrate is cleaved extremely well by Factor Xa, but not at all by Thrombin.
EnSens Factor Xa kit was challenged with purified active Factor Xa protease or purified active Thrombin protease. The assay was run in 384-well plates in a reaction volume of 20 uL. Excitation 625-635nm and Emission 660-670nm settings were used on a Tecan Infinite M1000 fluorescent plate reader and readings were taken every 2 minutes over 2 hours.
•EnSens® Factor Xa Activity Detection Substrate
•Far Red Fluorogen (FRF dye)
•Pre-activated substrate (EnSens®-PA, substrate positive control)
•2X Assay buffer
•Molecular biology grade water
(Coagulation factor Xa, FXa, prothrombinase, prothrombase, thrombokinase, thromboplastin, Stuart factor, Stuart-Prower factor)
A clotting cascade protease initially expressed as inactive proenzyme and activated by other coagulation factor proteases, factor Xa cleaves and activates prothrombin in the presence of factor Va. Factor Xa is inactivated by the serpin ZPI (protein Z-dependent protease inhibitor). It is the target of recent drugs rivaroxaban (XARELTO); apixaban (Eliquis); enoxaparin (Lovenox); edoxaban (Lixiana); betrixaban (LY517717); darexaban (YM150); TAK-442; and eribaxaban (PD0348292).
Together, factor Xa and thrombin proteases play prominent roles in the coagulation cascade and are major targets for emerging anti-coagulant therapies and associated diagnostics. EnSens fluorimetric reagents enable selective tracking and quantification of Factor Xa and Thrombin activation in purified protease, plasma, and whole blood samples.
EnSens Factor Xa Activity Detection Kit
- Product Code: 032014_07
- Availability: In Stock